Comparative study of tissue culture response of some selected basmati rice cultivars of Pakistan

Khalid Mehmood, Muhammad Arshad, Shaukat Ali, Mazher Qayyum, Ghulam Muhammad Ali


This study was conducted to select the best tissue culture responsive basmati rice cultivar among Basmati 370, Basmati 385, Super basmati and Shaheen basmati. N6 and MS media having four levels of 2, 4-D (1.5, 2.0, 2.5 and 3.0 mg/l) and three levels of agar (4.0, 5.0 and 6.0 g/l) were used in order to evaluate the most appropriate level required for calli formation. Basmati 370 showed the best response as compared to other cultivars on both MS and N6 media. The most cultivars behaved well for callus induction at N6 media, 5.0 g/l agar and 2.0-2.5 mg/l 2, 4-D. Basmati 370 showed callus formation; 74.0-85.07%, Super basmati showed 65.5-75.17%, Basmati 380 showed 72-79% and Shaheen basmati showed 53.0-67.87% callus formation. For regeneration, various treatments (0.5/2.0, 1.0/4.0, 1.5/5.0 mg/l) of NAA/BAP were used. Super basmati exhibited the best response for regeneration (58.33%) at 1.0/5.0 NAA/BAP. Based on our findings, Super basmati was found to be the best tissue culture responsive cultivar. These results will be helpful in unveiling many aspects of callus induction and regeneration and have potential use in genetic improvement of rice by employing various techniques of genetic transformation including Agrobacterium-mediated transformation and gene gun method.


Basmati 370; Basmati 385; Callus; Regeneration; Super basmati; Shaheen basmati

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Abbasi, S. S., Tahir, A., Raza, I., Abid, S., & Khan, M. N. (2015). Trend analysis and forecasting of wheat and rice in Pakistan. Pakistan Journal of Agricultural Research, 28(3), 310-317.

Ahmed, U. I., Ying, L., Bashir, M. K., Iqbal, M. A., Rizwan, M., Iqbal, M. M., Qamar, M. R., & Nazeer, A. (2015). Spatial price transmission in Pakistan: The case of wheat and rice markets. Pakistan Journal of Agricultural Research, 28(4), 354-362.

Akhter, M., Ali, M. A., Haider, Z., & Muzammil, S. (2015). Physico-chemical changes in grains of some advance lines/varieties of rice (Oryza sativa L.) after parboiling. Pakistan Journal of Agricultural Research, 28(2), 110-117.

Ali, S., Zhong, X. Q., & Yin, Z. X. (2004). Assessment of various factors involved in the tissue culture system of rice. Rice Science, 11, 345-349.

Bano, S., Jabeen, M., Rahim, F., & Ilahi, I. (2005). Callus induction and regeneration in seed explants of rice (Oryza sativa CV. Swat-II). Pakistan Journal of Botany, 37(3), 829-836.

Chu, C. C. (1978, May). The N6 medium and its applications to anther culture of cereal crops. In: Proceedings of symposium on plant tissue culture (pp. 45-50). Peking, China: Science Press.

Joyia, F. A., & Khan, M. S. (2013). Scutellum-derived callus-based efficient and reproducible regeneration system for elite varieties of indica rice in Pakistan. International Journal of Agriculture and Biology, 15, 27‒33.

Kajala, K., Covshoff, S., Karki, S., Woodfield, H., Tolley, B. J., Dionora, M. J. A., Mogul, R. T., Mabilangan, A. E., Danila, F. R., Hibberd, J. M., & Quick, W. P. (2011). Strategies for engineering a two-celled C4 photosynthetic pathway into rice. Journal of Experimental Botany, 62(9), 3001-3010.

Kalaiarasi, K., Sangeetha, P., Subramaniam, S., & Venkatachalam, P. (2014). Development of an efficient protocol for plant regeneration from nodal explants of recalcitrant bamboo (Bambusa arundinacea Retz. Willd) and assessment of genetic fidelity by DNA markers. Agroforestry Systems, 88(3), 527–537.

Long-ping, Y. (2014). Development of hybrid rice to ensure food security. Rice Science, 21(1), 1−2.

Mahmood, I. A., Ali, A., Shahzad, A., Asif, M., Ghumman, M., Arshad Ullah, M., Sultan, T., & Zaman, B. (2016). Crop residues and phosphorus effect on yield and economics of direct seeded rice and wheat grown under saline-sodic soil. Pakistan Journal of Agricultural Research, 29(1), 25-34.

Mehmood, K., Arshad, M., Ali, G. M., & Razzaq, A. (2013). Tissue culture responses of some wheat (Triticum aestivum L.) cultivars grown in Pakistan. Pakistan Journal of Botany, 45, 545-549.

Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiologia Plantarum, 15, 472-493.

Noor, A., Rashid, H., Chaudhry, Z., & Mirza, B. (2005). High frequency regeneration from scutellum derived calli of basmati rice cv. Basmati 385 and Super basmati. Pakistan Journal of Botany, 37(3), 673-684.

Pons, M. J., Marfa, V., Mele, E., & Massager, J. (2000). Regeneration and genetic transformation of Spanish rice cultivars using mature embryo. Euphytica, 114, 117-122.

Rabbani, M. A., Masood, M. S., Shinwari, Z. K., & Yamaguchi-Shinozaki, K. (2010). Genetic analysis of basmati and non-basmati Pakistani rice (Oryza sativa L.) cultivars using microsatellite markers. Pakistan Journal of Botany, 42(4), 2551-2564.

Rashid, H., Bokhari, S. N. R., Chaudhry, Z., & Naqvi, S. M. S. (2003). Studies on genotype response to callus induction from three basmati cultivars of rice (Oryza sativa L.). Pakistan Journal of Biological Sciences, 6(5), 445-447.

Rashid, H., Bokhari, S. Y. A., & Quraishi, A. (2001). Callus induction, regeneration and hygromycin selection of rice (Super basmati). Online Journal of Biological Sciences, 1(12), 1145-1146.

Rashid, H., Toriyama, K., Quraishi, A., Hinata, K., & Malik, K. A. (2000). An improved method for shoot regeneration from calli of indica rice (Basmati). Pakistan Journal of Biological Sciences, 3(12), 2229-2231.

Rashid, H., Yokoi, S., Toriyama, K., & Hinata, K. (1996). Transgenic plant production mediated by Agrobacterium in indica rice. Plant Cell Reports, 15, 727-730.

Shah, S. H., Ali, S., & Ali, G. M. (2013). A novel approach for rapid in vitro morphogenesis in tomato (Solanum lycopersicum Mill.) with the application of cobalt chloride. European Academic Research, 1, 2702‒2721.

Shah, S. H., Ali, S., Jan, S. A., & Ali, G. M. (2014a). Assessment of carbon sources on in vitro shoot regeneration in tomato. Pakistan Journal of Agricultural Sciences, 51, 197‒207.

Shah, S. H., Ali, S., Jan, S. A., Din, J., & Ali, G. M. (2014b). Assessment of silver nitrate on callus induction and in vitro shoot regeneration in tomato (Solanum lycopersicum Mill.). Pakistan Journal of Botany, 46, 2163‒2172.

Shah, S. H., Ali, S., Jan, S. A., Din, J., & Ali, G. M. (2015a). Callus induction, in vitro shoot regeneration and hairy root formation by the assessment of various plant growth regulators in tomato (Solanum lycopersicum Mill.). Journal of Animal and Plant Sciences, 25, 528‒538.

Shah, S. H., Ali, S., Jan, S. A., Din, J., & Ali, G. M. (2015b). Piercing and incubation method of in planta transformation producing stable transgenic plants by overexpressing DREB1A gene in tomato (Solanum lycopersicum Mill.). Plant Cell, Tissue and Organ Culture, 120, 1139–1157.

Shah, S. H., Ali, S., Hussain, Z., Jan, S. A., Din, J., & Ali, G. M. (2016). Genetic improvement of tomato (Solanum lycopersicum) with AtDREB1A gene for cold stress tolerance using optimized Agrobacterium-mediated transformation system. International Journal of Agriculture and Biology, 18, 471‒482.

Shakoor, U., Saboor, A., Baig, I., Afzal, A., & Rahman, A. (2015). Climate variability impacts on rice crop production in Pakistan. Pakistan Journal of Agricultural Research, 28(1), 19-27.

Sharan, V., Yadav, R. C., Yadav, N. R., & Chapagain, B. P. (2004). High frequency plant regeneration from desiccated calli of indica rice (Oryza sativa L.). African Journal of Biotechnology, 3, 256-259.

Woodward, A. W., & Bartel, B. (2005). Auxin: regulation, action, and interaction. Annals of Botany, 95, 707–735.

Zahra, N., Akmal, N., Naheed, S., Habib, N., Siddiqui, S., & Raza, I. (2015). Trend analysis of rice area and yield in Punjab. Pakistan Journal of Agricultural Research, 28(4), 439-444.

Zaidi, M. A., Narayanan, M., Sardana, R., Taga, I., Postel, S., Johns, R., Mcnulty, M., Mottiar, Y., Mao, J., Loit, E., & Altosaar, I. (2006). Optimizing tissue culture media for efficient transformation of different indica rice genotypes. Agronomy Research, 4(2), 563-575.


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